Le squadre partecipanti alla Persian Gulf Pro League 1399/1400 (2020/21), in ordine alfabetico.
- Aluminium Arak
- Esteghlal
- Foolad Khuzestan
- Gol Gohar Sirjan
- MachineSazi
- Mes Rafsanjan
- Naft Masjed Soleiman
- Nassaji Mazandaran
- Padideh Shahr Khodro
- Paykan
- Persepolis
- Saipa
- Sanat Naft
- Sepahan
- TractorSazi
- Zob Ahan